蒋佳明¹，曾燕琼¹，袁隽洁¹，邓晓东^2，3，景联培¹，邓 林¹，张薇薇^1*

（1.成都医学院公共卫生学院，四川成都 610500；

2.四川国际旅行卫生保健中心（成都海关口岸门诊部），四川成都 610041；

3.口岸疫病疫情监测四川省重点实验室，四川成都 610041）

摘要：研究飞燕草素（Delphinidin，Dp）抑制HER-2^＋乳腺癌细胞（MDA-MB-453）和HER-2-乳腺癌细胞（MCF-7）上皮细胞-间充质转化（EMT）效应。将不同浓度Dp分别处理MDA-MB-453和MCF-7细胞48 h，于倒置显微镜观察细胞形态及数量变化后，用蛋白质免疫印迹法和逆转录聚合酶链反应检测 EMT 标志性蛋白 E-cadherin、N-cadherin、Vimentin、Fibronectin 及关键调控因子锌指转录因子（Snail 1）表达。80 μmol/L 和 100 μmol/L 的Dp 能使MDA-MB-453 和 MCF-7 乳腺癌细胞的E-cadherin 标志蛋白表达上调，而降低与细胞迁移和侵袭能力有关的N-cadherin、Vimentin、Fibronectin及Snail因子表达水平。Dp体外具有抗MDA-MB-453和MCF-7乳腺癌EMT的效应，研究结果可为植物黄酮类化合物抗乳腺癌转移及辅助治疗提供新支撑。

关键词：飞燕草素；乳腺癌；人表皮生长因子受体-2；上皮细胞-间充质转化

中图分类号：R285.5    文献标识码：A    文章编号：1674-506X（2022）01-0023-0007

Research on the Resistant Effect on EMT of Breast Cancer with Delphinidin in vitro

JIANG Jia-ming¹, ZENG Yan-qiong¹, YUAN Jun-jie¹, DENG Xiao-dong², ³, JING Lian-pei¹, DENG Lin1, ZHANG Wei-wei^1*

（1.School of Public Health, Chengdu Medical College, Chengdu Sichuan 610500, China;

2.Sichuan International Travel Healthcare Center （Outpatient Department of Chengdu Customs Port）, Chengdu Sichuan 610041, China;

3.Key Laboratory of Sichuan Province for Port Disease Surveillance, Chengdu Sichuan 610041, China）

Abstract：In order to study the inhibitory effect on epithelial mesenchymal transition (EMT) of HER-2^＋ breast cancer cells (MDA-MB-453) and HER-2-breast cancer cells (MCF-7) with delphinidin (Dp), MDA-MB-453 and MCF-7 cells were treated with different concentrations of Dp for 48 h, respectively. The morphological and quantitative changes of cells were observed by inverted microscope. The expression of EMT marker protein E-cadherin, N-cadherin, Vimentin, Fibronectin and the key regulatory factor zinc finger transcription factor (Snail 1) were detected by western blotting and reverse transcription polymerase chain reaction. The results showed that 80 μmol/L and 100 μmol/L Dp could increase the expression of E-cadherin marker protein in MDA-MB-453 and MCF-7 breast cancer cells, but reduce the expression level of N-cadherin, Vimentin, Fibronectin and Snail related to cell migration and invasion. Therefore, Dp had been proved to have the resistant effect on EMT of MDA-MB-453 and MCF-7 breast cancer cells in vitro. The research results could provide the new support for the anti metastasis and adjuvant therapy of plant flavonoids.

Keywords：Delphinidin; breast cancer; human epithelial growth factor receptor-2; epithelial mesenchymal transition

doi：10.3969/j.issn.1674-506X.2022.01-004

收稿日期：2021-03-17

基金项目：四川省教育厅川菜发展研究中心科研项目（CC19Z28）；四川省中医药管理局中医药科学技术研究专项（2016Q074）；成都医学院2017年省级大学生创新训练计划项目（201713705064）；四川省科技计划项目（2015JY0034）；成都医学院2021年省级大学生创新创业训练计划项目（S202113705083）

作者简介：蒋佳明（1999-），男，本科。研究方向：营养与食品卫生学。

*通信作者：张薇薇（1985-），女，博士，副教授。研究方向：营养与食品卫生学。

引用格式：蒋佳明,曾燕琼,袁隽洁,等.Delphinidin抗乳腺癌EMT体外效应研究[J].食品与发酵科技,2022,58(1):23-28,41.